![]() ![]() It was found that human respiratory viruses uniquely bind 9- O-acetylated α2,8-linked disialoside found on glycosphingolipids 21.ĭespite advances, the roles of distinct O-acetylated sialosides in health and disease remain difficult to explore. Recent analysis of receptor specificities of these viruses infecting different species of animals and humans demonstrated host-specific patterns of receptor recognition in relationship to both the pattern of acetylation and glycosidic linkage type. O-acetylated sialic acids serve also as receptors for many viruses including embecoviruses (family Coronaviridae), toroviruses (Tobaniviridae), and influenza C and D viruses (Orthomyxoviridae) 21. In addition, it can block the activity of bacterial sialidases and by this means protect the integrity of the epithelial mucus barrier. It also substantially reduces the rate of hydrolysis by several human endogenous sialidases thereby regulating properties of glycoconjugates such as turnover and degradation 20. O-acetylation can preclude recognition by glycan binding proteins such as the Siglec immune-receptors and complement protein factor H and as a result can function as a molecular switch 19. Furthermore, there are marked differences in the expression of sialoglycans in different species, which likely is due to evolutionary pressure evoked by host–pathogen interactions 14.Ī growing body of literature associates sialic acid O-acetylation with various diseases including cancer, immune disorders, and infection 15, 16, 17, 18. The expression of sialic acids is regulated in a developmental and tissue-specific manner. The resulting glycotopes can be presented at different underlying glycan moieties and can be part of Asn- and Ser/Thr-linked glycans ( N- and O-linked glycans, respectively), glycolipids as well as free floating glycans such as human milk oligosaccharides. ![]() Further structural diversity comes from different Neu5Ac and Neu5Gc glycosidic linkage types and the most common ones are α2,3-linked to galactose (Gal), α2,6-linked to Gal and N-acetyl-galactosamine (GalNAc) and α2,8-linked to another sialic acid. They can be modified by acetyl esters at the 4-, 7-, 8-, and/or 9-position to give as many as 15 different patterns of O-acetylation 13. N-Acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) are major forms of sialic acid expressed by mammals. Sialylation of glycans also modulates half-life, immunogenicity, and properties of biologicals 10, 11, 12. Sialoglycans regulate many biological and disease processes 6, 7, 8, and can function as receptor for many pathogens including viruses, bacteria and protozoa 9. ![]() Several pathogenic microorganisms also express sialylated glycoconjugates which are used for molecular mimicry to evade host immune detection 3, 4, 5. Sialic acids are negatively charged nine-carbon monosaccharides that are often part of complex glycans of higher animals 1, 2. It uncovered contrasting sialic acid linkage types of acetylated and non-acetylated sialic acids and provided a rationale for sialic acid binding preferences of equine H7 influenza A viruses. The CCS values were used to characterize O-acetylated sialosides from mucins and N-linked glycans from biologicals as well as equine tracheal and nasal tissues. It is based on the use of a library of synthetic O-acetylated sialosides to establish intrinsic collision cross section (CCS) values of diagnostic fragment ions. Here, we describe a drift tube ion mobility-mass spectrometry approach that can elucidate exact O-acetylation patterns as well as glycosidic linkage types of sialosides isolated from complex biological samples. A lack of analytical methods that can determine exact structures of sialic acid variants is a hurdle to determine roles of distinct O-acetylated sialosides. O-acetylation is a common modification of sialic acids that has been implicated in a multitude of biological and disease processes. ![]()
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![]() ![]() ![]() You also have the typical late night TV movie special effects which are not believable and alongside them you have an abundance of tongue in cheek dialogue. As such you know what to expect to a certain extent with scenes of running from avalanches, sustaining injuries, arguments between the family and so on. So in many ways "Ice Quake" is the combination of two types of movies as we have the family stuck in the wilderness but then combined with a threat of global disaster with all these natural phenomena such as ice geysers, shockwaves and avalanches. The thing is that these opening scenes are indicative of "Ice Quake" as the movie is predictable but with some amusing elements. But there is more because lo and behold a crack forms in the snow between them, something which you may have seen in a ton of other disaster movies but here we get a bit of a switch up on the obvious, a frankly predictable one as one of the men leaps across the crevice. The opening of "Ice Quake" is either going to make you smile or grimace as we see Santa on a snowmobile! It turns out Santa is actually part of a military geologist team who was meant to be playing Santa at a community event but has had to head up to the mountain to place some sensors with a colleague. But it is not just the Webster's who are in danger as a methane explosion threatens to destroy the planet unless they, with the help of Michael's superior, Colonel Bill Hughes ( Victor Garber - The Music Man), can stop it. But it seems that Shane's eagerness may come in useful when due to an ice shelf collapsing in the Russian Arctic shockwaves from the Ice Quake hit the region and lead to a whole series of natural disasters which put the Webster family in deadly danger. The only one who is up for the holiday stuff is their young son, Shane (Ryan Grantham), who, when they go into the Alaskan wilderness, packs enough gear for a fortnight. It's Christmas Eve and Emily Webster ( Holly Elissa - Polar Storm) wants the family to do some traditional stuff, not always easy as her husband, Michael ( Brendan Fehr - Wrapped Up In Christmas), is a military geologist who doesn't know the meaning of "day off" and their teenage daughter, Tia ( Jodelle Ferland - Pictures of Hollis Woods), would rather be with her friends than her parents. ![]() ![]() ![]() This tool is relatively fast and takes a few minutes to provide accurate information about a caller. 1.2 Why FindPeopleFast Stand Out From The Crowd? The site also includes public records of two types, including Individual Records and Government Agencies and Institutions. FindPeopleFast features people search, phone lookup, and email lookup elements. Part 1: FindPeopleFast 1.1 What Is FindPeopleFast?įindPeopleFast is a cell phone reverse lookup tool that uses an advanced system of algorithms to verify the identity of people you search online. 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It is quick, simple and quite frankly the best there is out there! ” “ NumLookup is by far the most accurate phone number lookup service I have used to date. ![]() ![]() ![]() ![]() Like I said, he’s a dickhead.Īdrian’s plan is complicated, so I’m going to give you the short version, followed by the longer-but-still short version. Manhattan is intrigued by the random, unpredictable chaos that brings each human life into being, and decides to return with Laurie to Earth to help stop the war. The news devastates and confuses her, as she struggles to reconcile how her mother could’ve gone on to have a romantic relationship with the man who had brutally attacked her years before. ![]() Manhattan, and partially using his ability to see past events as clearly as the present, Laurie realizes that the Comedian is her father, and that he and her mother loved each other. Laurie goes with him back to Mars to try and convince him to help them stop the impending nuclear war and whatever sinister conspiracy seems to be egging it on.ĭuring her talk with Dr. Manhattan reappears and admits that he can’t really find a reason why he should care about humanity or what happens to the world, because in addition to being an immortal metahuman, he is also a colossal douche canoe. They bust Rorschach out of prison, at which point Dr. (The informant, a former supervillain named Moloch, is murdered before Rorschach arrives.)ĭan and Laurie get together, and begin to suspect that some aspects of Rorschach’s theory may be accurate. Meanwhile, an assassin shows up at Adrian’s office and tries to kill him, and Rorschach finally gets arrested after a meeting with an informant turns out to be a setup. With him gone, the Soviet Union takes the opportunity to invade Afghanistan, bringing the world even closer to nuclear war. Manhattan, whose god-like status already has him struggling to continue to care about regular humans, has a good old fashioned freakout and teleports to Mars, like you do. Manhattan gets hit with a series of allegations from former friends and coworkers claiming that exposure to his super-powered body gave them all terminal cancer. Particularly unfazed is Laurie, because the Comedian had assaulted and nearly raped her mother, the original Silk Spectre, back when her mother and the Comedian were on the same superhero team, the Minutemen. Manhattan of both the Comedian’s death and of his “superhero killer” theory, but none of them take his warning very seriously. Rorschach investigates, initially believing that someone is bumping off all the old superheroes. The story begins with the Comedian getting murdered by an unknown attacker. We’re also led to believe that he smells like a sack of dead cats in a diaper bin. He’s still prowling the streets, attacking criminals and evading capture by the police. The only hero who refuses to retire is Rorschach, a brutal, murderous vigilante clad in a fedora and a white mask covered in ink blot patterns that are constantly shifting. Dan is kind of a sad Batman, while Laurie is sort of like Black Widow meets Black Canary (again, without any superpowers). Manhattan in a government facility as his lover / babysitter. Dan and Laurie’s identities remain secret, although Laurie lives with Dr. Adrian retires publicly, revealing his identity to the world. The rest of the heroes – Dan Dreiberg AKA Nite Owl, Laurie Jupiter AKA Silk Spectre, and Adrian Veidt AKA Ozymandias – are forced to retire. Manhattan and The Comedian – a violent, Punisher-type militant – are allowed to continue to operate, because they agree to work exclusively as agents of the U.S. ![]() In the story’s timeline, superheroes become outlawed in the 1970s. ![]() ![]() ![]() Some people have commented on the (relative) difficulty in finding Halo Top at stores. That involved not only experimentation with ingredients, but also some really technical stuff as it related to machinery. That was another “uh oh” moment, another huge setback that we had to work through. It was like a movie-I heard things like “give it up, it can’t be done, kid.” We finally found one local manufacturer who was willing to work with us, and sure enough, we actually blew their pipes. None of the bigger facilities wanted to do it. Luckily I ended up with something that was enough to get into stores, have some success, secure distribution, but there finally came a time to scale up to the big leagues. It was tiring but also exhilarating trying to crack the code. Honestly that period alone took months, and I was still practicing law at the time on top of it. So there was a ton of experimentation at that point in scaling up the recipe-which ingredients worked and didn’t work, how to replace the sugar in the ice cream (which not only sweetens, but provides body and texture), et cetera. In fact, the first time I ran my scaled-up recipe in a commercial ice cream facility was such a disaster that I had an “uh oh” moment. Making large quantities on serious equipment is a whole different ballgame. And I quickly learned that taking your kitchen recipe and just multiplying it by 100 does not work. So I first had to scale up my little kitchen recipe. ![]() But after realizing it tasted pretty good in my kitchen, and being absolutely miserable practicing law, I decided to take the plunge, quit law, and give Halo Top a real shot, because even in my kitchen it was really good. (I can’t eat a lot of sugar without feeling weird, so I liked to tool around with different foods and sugar replacements on my own.) I was practicing law at the time, and I’d honestly just bought a Cuisinart machine from Amazon to play around with. Was there a long period of trial and error with Halo Top? What was that process like?įirst, Halo Top began because I was making it in my kitchen for myself without even thinking of bringing it to market. Think about it: how many lower-calorie products use top-notch ingredients? And it really does help the taste-there was a noticeable difference when we experimented with cheap ingredients in the early days. Halo Top is good ice cream because, despite it being a “better-for-you” food, we use extremely top-shelf ingredients (e.g., organic lemon peel, real vanilla, etc.) That’s oddly rare in this “low-cal” category and I think we were the first company to take that route. I'm sure you don't want to reveal too much of your secret, but what can you tell me about Halo Top's approach that allows for a satisfying ice cream? How do they make it taste so good? Why can't I find it at my local store? We spoke with CEO and founder Justin Woolverton via email to find out. But it also presents us with a set of questions. So it seems that the L.A.-based company has it all lined up. ![]() Half of the flavors contain less than 300 calories in an entire pint the highest count comes in at 360.Ĭheck out a video from one of their California packaging factories: All this has conspired for a perfectly guilt-free indulgence. ![]() Part of the secret, as noted at Bloomsberg, is a no-calorie sugar alcohol called erythritol, a zero-calorie sweetener called Stevia, as well as milk protein to make up for the omitted fat (which, aside from other things, helps ice cream maintain its scoopable texture). And the "red velvet" is similarly rich, giving us a full-bodied profile that's replete with cocoa and brownie dough. The "peanut butter cup" is unabashedly sweet with the strong undertow of, what else, peanut butter. And the flavors-covering a broad range from "vanilla bean" to "birthday cake"-are strikingly bold. There's the requisite milkiness-yes, there's dairy in it, as well as other basics like eggs. But we did sample the dessert, and we're happy to report that this supposed ambrosia tastes.exactly like ice cream. We weren't quite as bold we didn't subsist on Halo Top for over a week, nor did we hook ourselves up to an IV bag filled with ice cream. And when one GQ writer ate nothing but Halo Top for 10 days straight, he actually lost about 10 pounds by the end of his experiment. BuzzFeed, in one of their patented people-trying-things posts, gave Halo Top glowing reviews for its taste. An entire pint of ice cream that clocks in at less than 400 calories? One that's low on carbs, but relatively high in protein? This all sounded like a bunch of alternative facts, before the term was even coined. The L.A.-based Halo Top made waves in 2016. ![]() ![]()
![]() PLEASE NOTE THAT THE LICENSOR STRONGLY RECOMMENDS THAT YOU TRY THE FREE TRIAL VERSION OF THE SOFTWARE BEFORE PURCHASING TO ENSURE THAT THE SOFTWARE SATISFIES YOUR REQUIREMENTS. IF YOUR RETAILER DOES NOT ACCEPT THE RETURN THEN PLEASE CONTACT THE LICENSOR. IF YOU DO NOT AGREE TO THE TERMS OF THIS LICENCE THEN PLEASE DELETE THE SOFTWARE OR RETURN THE SOFTWARE AND THE RELATED DOCUMENTATION TO YOUR RETAILER. IF YOU USE THIS SOFTWARE THEN YOU AGREE TO BE BOUND BY THE TERMS OF THIS LICENCE AGREEMENT. PLEASE READ THIS LICENCE AGREEMENT CAREFULLY BEFORE USING THIS SOFTWARE. THIS IS A SOFTWARE LICENCE AGREEMENT BETWEEN LITERATURE AND LATTE LIMITED (registered in England and Wales with number 6240207 whose registered office is at Lowin House Tregolls Road Truro Cornwall TR1 2NA) (the ‘LICENSOR’) AND YOU. It won’t tell you how to write-it just provides everything you need to start writing and keep writing. Scrivener’s users include best-selling novelists, screenwriters, students, academics, lawyers, journalists and translators. You can even share using different formatting, so that you can write in your favorite font and still keep your editor happy. Once you’re ready to share your work with the world, turn your manuscript into a beautiful ebook or PDF, export to Word, or simply print it out. Or check for consistency against an earlier chapter. Write a description based on a photograph. Need to refer to research? In Scrivener, your background material is always at hand, and you can open it right alongside what you’re working on. All text sections in Scrivener are integrated with an easy-to-use project outline, so working with an overview of your manuscript is only ever a click away, and turning Chapter Four into Chapter One is as simple as drag and drop. Whether you plan or plunge, Scrivener works your way: hammer out every last detail before typing a word, or carve out a draft and restructure later. Got a great idea but don’t know where it fits? Compose your text in any order you want-write when inspiration strikes and find its place later. At its heart is a simple ring-binder metaphor-gather material and switch between writing, notes and research with ease. ![]() Tailor-made for creating long manuscripts, Scrivener lets you break up your writing into sections as small or large as you like. Scrivener combines all the writing tools you need to craft your first draft, from nascent notion to final full stop. At any point during the trial period you will be able to make an in-app purchase to license Scrivener. ** Please note: Scrivener comes with a 30 days of use free trial. ![]() ![]() ![]() ![]() You may need to adjust upon reheating as well, since the pasta absorbs some of the liquid and flavor as it sits. Sea salt + fresh cracked pepper – after the soup has finished cooking, taste and adjust the seasoning to your preference.You’ll need 2 tablespoons of freshly squeezed lemon juice from one lemon. I recommend buying organic chicken when possible. Organic Chicken – Rotisserie chicken makes this soup come together quickly.For a more protein-packed soup, use organic chicken bone broth which typically has 10 gram of per cup! You can get by with 4 or one 32 oz container, if needed. Organic Chicken broth – you’ll need 6 cups of broth for this recipe.Adding a half teaspoon to the soup is an easy way to sneak in some turmeric in your diet, especially in the colder months when your immune system could use a boost. Turmeric – turmeric has healing properties and can help reduce inflammation.Fresh is best but feel free to use dried herbs. Rosemary + thyme – classic chicken soup flavor.Veggies – onion, carrots, and garlic get sautéed in heart-healthy olive oil.Avocado with sourdough toast would be a lovely addition. I This cozy, healing soup is one you’ll make over and over again throughout the colder months.Įven though this soup is lower in carbohydrates, I still recommend balancing it out with additional carbohydrates and healthy fats. Turmeric has anti-inflammatory, healing properties that are especially beneficial during the colder months. Now, what makes this soup really stand out is the addition of turmeric and lemon to the herbed chicken broth. It’s nourishing and will warm you from the inside out just like a bowl of soup should. It’s a lower carb meal that packs in the protein and veggies and happens to be gluten-free, grain-free and paleo-friendly if that’s important to you. Your favorite classic soup but without noodles. Grain-free, Gluten-free and paleo friendly.Ī classic with a twist! This Chicken No Noodle Soup is exactly what is sounds like. It’s a veggie-loaded chicken no noodle soup with herbs and a touch of turmeric and lemon. A nourishing chicken soup with no noodles that comes together quickly using rotisserie chicken. ![]() ![]() ![]() They have a well stocked salsa and fixings bar, and I noticed many people who looked like regulars were being generous with the chopped lettuce, cucumbers, carrots, limes, and salsas. The tacos were all good, but my favorite was the carne asada. 3 tacos, a side of refried beans, and a Coronita was under $10. I haven't experienced that at a order-at-the-counter restaurant before, and almost forgot to pay when I left. And you don't pay until you leave the restaurant - they keep your ticket with your name on it at the counter so you can settle up on the way out. For example, there isn't a clear line for ordering, just a broad counter that you approach then give your order to one of the friendly guys behind the counter. country and don't know all the procedures. Their are a few quirks about this place that keep you on your toes - as if you are visiting a restaurant in another. If you want to try street tacos in a clean, somewhat familiar environment, this is a great place to do that. Wish we had one here in town because we'd hit it every weekend ) definitely will be back. Fed our family of 7 and had leftovers for dinner. ![]() Our hotel heated up the meat and warned our tortillas for us the next day and we ate it on the road. Love this place, fast, efficient, fair in price.Īnd what do you know. Wrapped it all up for me, got our food in 15 minutes and bagged it all to seal it in for the next day. Loved how they just let me pack it all in and then some. We had a funny incident where everyone in front of us plus us thought there was a drive thru here and got all backed up by a parking car so we all had to slowly reverse out n repark lol that took up a bit of time but we went in ordered our carne asada family size meal and extra tortillas and again they gave us all the stuff we need to pack to the brim their amazing salsas, Pico de gallo, fresh salad fixings which they kept restocking. Even at late night this place was hopping. So in this case wow thanks Ta Carbon again for the most yummy lunch on the road. Yes I did wonder if it will taste less fresh but when something is made fresh the night before, it stays fresh the next day :) We almost didn't make it because we weren't in this city long enough but my brilliant thinking decided to hit here late at night to take out for the next days lunch on the road to the Grand Canyon. ![]() How can I forget? This time we were closer to this location and had to find a way to get their carne asadas to-go. Thank you hubby for reminding me that Ta Carbon was in this city last time we were on a road trip. Enjoy and remember you were a first timer before - so bring a rookie! More If you want to buy or give it’s up to you but no pressure.ħ. Don’t feel obligated to buy from the vendors that walk around inside. Don’t be intimidated as the guys working and customers are awesome and will help if needed - most speak English.Ħ. ![]() Their parking lot is small - but you’ll see ad Hoc parking as it fills up.ĥ. Go to left side of counter to order (right side is for when you pay before you leave). Then, to snap them out of food coma before sending them back to their offices, I get them the best horchata I’ve ever had (less sweet and more flavor than most) and remind them that they are buying next time!ġ. The grind and smiles make it all worth it. When I bring the food I have them visit the salsa bar and get one of each to try them all. I get the 1 pound of meat ( half Asada and half Pastor) flower and corn tortillas and a side of their amazing beans and a bottle of water. meat right!! I am a professional and I work 3 blocks from here and love to see the facial expressions on my clients AND prospects when I bring them here - point to them to sit down. ‘Nuff said!”įolks think of the best street taco you ever had from a cart (not the fancy gourmet stuff) and multiply the quality by 2 and quantity by 4 - with tables! 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The main antioxidants in regular and decaf coffee are hydrocinnamic acids and polyphenols ( 1, 12).Īntioxidants are very effective at neutralizing reactive compounds called free radicals. ![]() This difference is most likely caused by a small loss of antioxidants during the decaffeination process. It’s actually the single biggest source of antioxidants in the Western diet ( 5, 6, 7).ĭecaf usually contains similar amounts of antioxidants as regular coffee, although they may be up to 15% lower ( 8, 9, 10, 11). Summary:ĭecaf coffee beans are washed in solvents to remove 97% of the caffeine content prior to roasting.Īside from the caffeine, the nutritional value of decaf coffee should be almost identical to that of regular coffee.ĭecaf coffee is loaded with antioxidants and contains nutrientsĬoffee is not the devil it’s been made out to be. This can make decaf coffee more pleasing to those who are sensitive to the bitter taste and smell of regular coffee. However, the taste and smell may become a little milder, and the color may change, depending on the method used ( 1). The nutritional value of decaf coffee should be almost identical to regular coffee, apart from the caffeine content. The beans are decaffeinated before they’re roasted and ground. Most of them include water, organic solvents, or carbon dioxide ( 1).Ĭoffee beans are washed in the solvent until the caffeine has been extracted from it, then the solvent is removed.Ĭaffeine can also be removed using carbon dioxide or a charcoal filter - a method known as the Swiss Water Process. There are many ways to remove caffeine from coffee beans. It’s coffee from coffee beans that have had at least 97% of their caffeine removed. ![]() |
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